Figure 7.
Variability in recovery from inactivation in WT and FGF14 KO cells: Some FGF14 KO CCs exhibit a residual component of slow recovery from inactivation.(A) Individual fractional recoveries for 16 WT CCs are shown based on the standard paired-pulse recovery at −80 mV. Red line shows best fit to averaged recoveries from WT cells. (B) The averaged recovery from the 33 cells (from 14 animals) in A, also shown in Fig. 6 F. Best fit of a double exponential: Af = 0.47 ± 0.01, τf = 7.0 ± 0.6 ms, As = 0.53 ± 0.02, τs = 584.6 ± 66.3 ms. (C) Paired-pulse recoveries for 33 total CCs from FGF14 KO mice (recovery at −80 mV). Time courses plotted in black were best fit with a single exponential recovery, while those in blue required two exponential components. Red is the best fit to averages of all cells. (D) For FGF14 KO recoveries, recovery curves are plotted for all cells together (red, identical to FGF14 KO in Fig. 6 F), cells fit with only a single exponential time course (green), and cells for which the recovery curve required two exponentials. For grouping of all cells (33 cells from 12 animals) as in Fig. 6 F, Af = 0.90 ± 0.01, τf = 7.9 ± 0.5 ms, As = 0.10 ± 0.05, τs = 1,129.4 ± 1,761.3 ms. For FGF14 KO cells in which only a single exponential was required, Af = 0.97 ± 0.01, τf = 7.2 ± 0.4 ms. For FGF14 KO cells in which recovery required two exponentials, Af = 0.76 ± 0.01, τf = 8.6 ± 0.5 ms, As = 0.24 ± 0.06, τs = 1,450.0 ± 956.6 ms. (E) Fractions of the fast recovery component for WT (gray) and FGF14 KO (red) were placed in 0.05-wide bins. Fast amplitude values for WT and FGF14 KO were compared by using a KS test because of the nonnormal distributions, yielding P = 0.000. (F) Mean ± SD and individual fast time constants are shown for WT and FGF14 KO CCs with adjusted P > 0.9999 (ANOVA with Bonferroni multiple comparisons test). (G) Mean ± SD and individual slow time constants; for WT versus FGF14 KO, P < 0.0001.

Variability in recovery from inactivation in WT and FGF14 KO cells: Some FGF14 KO CCs exhibit a residual component of slow recovery from inactivation. (A ) Individual fractional recoveries for 16 WT CCs are shown based on the standard paired-pulse recovery at −80 mV. Red line shows best fit to averaged recoveries from WT cells. (B) The averaged recovery from the 33 cells (from 14 animals) in A, also shown in Fig. 6 F. Best fit of a double exponential: Af = 0.47 ± 0.01, τf = 7.0 ± 0.6 ms, As = 0.53 ± 0.02, τs = 584.6 ± 66.3 ms. (C) Paired-pulse recoveries for 33 total CCs from FGF14 KO mice (recovery at −80 mV). Time courses plotted in black were best fit with a single exponential recovery, while those in blue required two exponential components. Red is the best fit to averages of all cells. (D) For FGF14 KO recoveries, recovery curves are plotted for all cells together (red, identical to FGF14 KO in Fig. 6 F), cells fit with only a single exponential time course (green), and cells for which the recovery curve required two exponentials. For grouping of all cells (33 cells from 12 animals) as in Fig. 6 F, Af = 0.90 ± 0.01, τf = 7.9 ± 0.5 ms, As = 0.10 ± 0.05, τs = 1,129.4 ± 1,761.3 ms. For FGF14 KO cells in which only a single exponential was required, Af = 0.97 ± 0.01, τf = 7.2 ± 0.4 ms. For FGF14 KO cells in which recovery required two exponentials, Af = 0.76 ± 0.01, τf = 8.6 ± 0.5 ms, As = 0.24 ± 0.06, τs = 1,450.0 ± 956.6 ms. (E) Fractions of the fast recovery component for WT (gray) and FGF14 KO (red) were placed in 0.05-wide bins. Fast amplitude values for WT and FGF14 KO were compared by using a KS test because of the nonnormal distributions, yielding P = 0.000. (F) Mean ± SD and individual fast time constants are shown for WT and FGF14 KO CCs with adjusted P > 0.9999 (ANOVA with Bonferroni multiple comparisons test). (G) Mean ± SD and individual slow time constants; for WT versus FGF14 KO, P < 0.0001.

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