Figure 6.
FGF14 KO abolishes all or most slow recovery from inactivation, with little effect on fast recovery.(A) Standard paired-pulse recovery at −80 mV for a WT CC. (B) Paired-pulse recovery −80 mV for a CC from an FGF14 KO mouse. (C) Paired-pulse recovery at −120 mV for the cell shown in A. (D) Paired-pulse recovery at −120 mV for the FGF14 KO cell in B. (E) Time course of recovery from inactivation at −60 mV mV (means ± SD) for WT and FGF14 KO CCs (n indicates numbers of cells in all panels). Double exponential fit to WT recoveries was: Af = 0.29 ± 0.01, τf = 32.4 ± 2.7 ms, As = 0.49 ± 0.02, τs = 1336.4 ± 176.0 ms. Single exponential fit to FGF14 KO recoveries: Af = 0.48 ± 0.005 (normalized to full recovery at −80 mV), τf = 26.19 ± 1.07 ms. (F) Recoveries at −80 mV. This includes cells in addition to those in Fig. 2. For WT, Af = 0.47 ± 0.01, τf = 6.95 ± 0.59 ms, As = 0.53 ± 0.02, τs = 584 ± 66.3 ms. For FGF14 KO and single exponential fit (blue line), Af = 0.92 ± 0.01, τf = 8.40 ± 0.57 ms. For FGF14 KO and two exponential fit (red), Af = 0.90 ± 0.01, τf = 7.92 ± 0.46 ms, As = 0.10 ± 0.05, τs = 1,129.4 ± 1,761.3 ms. (G) At −100 mV, for WT, Af = 0.48 ± 0.02, τf = 4.38 ± 0.49 ms, As = 0.54 ± 0.02, τs = 335.3 ± 43.1 ms. For FGF14 KO, single exponential: Af = 1.02 ± 0.01, τf = 3.83 ± 0.32 ms; for double exponential, Af = 0.98 ± 0.02, τf = 3.55 ± 0.30 ms, As = 0.09 ±0.04, τs = 423.4 ± 650.3 ms. (H) At −120 mV, for WT, Af = 0.46 ± 0.02. τf = 2.17 ± 0.31 ms, As = 0.56 ± 0.02, τs = 83.3 ± 10.2 ms. For FGF14 KO, single exponential, Af = 0.99 ± 0.02, τf = 2.00 ± 0.28 ms; for double exponential, Af = 0.89 ± 0.03, τf = 1.69 ± 0.16 ms, As = 0.17 ± 0.03, τs = 115.9 ± 62.2 ms. (I) Mean (+SD) time constants for slow (τs) and fast (τf) recovery time constants from WT CCs and single exponential recovery time constants for FGF14 KO cells are plotted as a function of recovery voltage.

FGF14 KO abolishes all or most slow recovery from inactivation, with little effect on fast recovery. (A) Standard paired-pulse recovery at −80 mV for a WT CC. (B) Paired-pulse recovery −80 mV for a CC from an FGF14 KO mouse. (C) Paired-pulse recovery at −120 mV for the cell shown in A. (D) Paired-pulse recovery at −120 mV for the FGF14 KO cell in B. (E) Time course of recovery from inactivation at −60 mV mV (means ± SD) for WT and FGF14 KO CCs (n indicates numbers of cells in all panels). Double exponential fit to WT recoveries was: Af = 0.29 ± 0.01, τf = 32.4 ± 2.7 ms, As = 0.49 ± 0.02, τs = 1336.4 ± 176.0 ms. Single exponential fit to FGF14 KO recoveries: Af = 0.48 ± 0.005 (normalized to full recovery at −80 mV), τf = 26.19 ± 1.07 ms. (F) Recoveries at −80 mV. This includes cells in addition to those in Fig. 2. For WT, Af = 0.47 ± 0.01, τf = 6.95 ± 0.59 ms, As = 0.53 ± 0.02, τs = 584 ± 66.3 ms. For FGF14 KO and single exponential fit (blue line), Af = 0.92 ± 0.01, τf = 8.40 ± 0.57 ms. For FGF14 KO and two exponential fit (red), Af = 0.90 ± 0.01, τf = 7.92 ± 0.46 ms, As = 0.10 ± 0.05, τs = 1,129.4 ± 1,761.3 ms. (G) At −100 mV, for WT, Af = 0.48 ± 0.02, τf = 4.38 ± 0.49 ms, As = 0.54 ± 0.02, τs = 335.3 ± 43.1 ms. For FGF14 KO, single exponential: Af = 1.02 ± 0.01, τf = 3.83 ± 0.32 ms; for double exponential, Af = 0.98 ± 0.02, τf = 3.55 ± 0.30 ms, As = 0.09 ±0.04, τs = 423.4 ± 650.3 ms. (H) At −120 mV, for WT, Af = 0.46 ± 0.02. τf = 2.17 ± 0.31 ms, As = 0.56 ± 0.02, τs = 83.3 ± 10.2 ms. For FGF14 KO, single exponential, Af = 0.99 ± 0.02, τf = 2.00 ± 0.28 ms; for double exponential, Af = 0.89 ± 0.03, τf = 1.69 ± 0.16 ms, As = 0.17 ± 0.03, τs = 115.9 ± 62.2 ms. (I) Mean (+SD) time constants for slow (τs) and fast (τf) recovery time constants from WT CCs and single exponential recovery time constants for FGF14 KO cells are plotted as a function of recovery voltage.

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