Figure 2.
WT CaV1.1 does not elicit voltage-dependent fluorescence change when labeled with a thiol-reactive fluorophore.(A) Top view of a cryo-EM structure of the pore-forming α1S subunit of CaV1.1 channels (Protein Data Bank accession no. 5GJV; Wu et al., 2016), illustrating four VSDs that surround and control the central pore. (B) Simultaneously recorded Ba2+ currents (black) and fluorescence signals (gray traces) at the indicated membrane potentials from human CaV1.1 channels (α1S+β1a+Stac3) without an engineered Cys, expressed in Xenopus oocytes and labeled with thiol-reactive fluorophores, are shown.

WT CaV1.1 does not elicit voltage-dependent fluorescence change when labeled with a thiol-reactive fluorophore. (A) Top view of a cryo-EM structure of the pore-forming α1S subunit of CaV1.1 channels (Protein Data Bank accession no. 5GJV; Wu et al., 2016), illustrating four VSDs that surround and control the central pore. (B) Simultaneously recorded Ba2+ currents (black) and fluorescence signals (gray traces) at the indicated membrane potentials from human CaV1.1 channels (α1S+β1a+Stac3) without an engineered Cys, expressed in Xenopus oocytes and labeled with thiol-reactive fluorophores, are shown.

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