Figure 1.

Surface temperature measures from beating cardiomyocytes. (A) Paced cardiomyocytes. Superimposed data of Fluo4 (top), and EuTTA (red), Rh101 (blue), and EuTTA/Rh101 (black; bottom). Data obtained by 10 stimuli were superimposed. A calibration bar of 0.01°C. Arrows indicate time of stimulation (data from Fig. 4 D in Oyama et al. (2020)). (B) Measurement for CCCP-induced thermogenesis in rat neonatal cardiomyocytes. Time course of changes in the EuTTA/Rh101 ratio of ratiometric thermometer (RT) nanosheets with cardiomyocytes upon application of 10 µM CCCP (red) or 0.1% DMSO (black). Number of cells, 18 (n = 3) for both CCCP and DMSO. The horizontal black line indicates the period during which CCCP or DMSO was present. Data from Fig. S11 B in Oyama et al. (2020).

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