Figure 9.
The gating elements in TMEM16A control the anion selectivity. (A) I-V relationships for I641A/5Q and G644P/5Q mutant channels. Currents were recorded from cells exposed to the indicated anions (top left) and dialyzed with an internal solution without Ca2+. (B) The anion permeability ratios for 5Q (green), I641A/5Q (salmon), and G644P/5Q (yellow) channels. The permeability ratios of the mutant channels were calculated using the changes in reversal potentials obtained from cells dialyzed with a pipette solution without Ca2+ as shown in A. The permeability ratios were calculated under the following conditions: [X−]e/[Cl−]i = 140/40 mM and pHe = pHi = 7.3. **, PX/PCl values were statistically different (P = 0.01) from those of the 5Q channel at 0 Ca2+ (replotted from Fig. 6). The data were statistically analyzed using one-way ANOVA with a Tukey’s post-hoc test.

The gating elements in TMEM16A control the anion selectivity. (A) I-V relationships for I641A/5Q and G644P/5Q mutant channels. Currents were recorded from cells exposed to the indicated anions (top left) and dialyzed with an internal solution without Ca2+. (B) The anion permeability ratios for 5Q (green), I641A/5Q (salmon), and G644P/5Q (yellow) channels. The permeability ratios of the mutant channels were calculated using the changes in reversal potentials obtained from cells dialyzed with a pipette solution without Ca2+ as shown in A. The permeability ratios were calculated under the following conditions: [X−]e/[Cl−]i = 140/40 mM and pHe = pHi = 7.3. **, PX/PCl values were statistically different (P = 0.01) from those of the 5Q channel at 0 Ca2+ (replotted from Fig. 6). The data were statistically analyzed using one-way ANOVA with a Tukey’s post-hoc test.

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