![SCN−MFs induce AMF-like behavior on the apparent Ca2+sensitivity of WT TMEM16A. (A) Examples of macroscopic currents induced by applying increasing [Ca2+]i (indicated at the top of the panel) to the cytosolic side of four different inside-out patches held at +80 mV. The extracellular side of the patches was exposed to 0.25 (olive, n = 9), 0.5 (orange, n = 7), 0.75 (green, n = 5), or 1 (blue, n = 6) SCN− MF. [Cl−]i = 40 mM, pHe = pHi = 7.3. (B) Concentration–response curves constructed with the current magnitudes obtained with 0, 0.25, 0.5, 0.75, and 1 SCN− MF shown in A. Continuous lines are fits of Hill equation, used to determine EC50 and nH coefficient. (C) Relationship between SCN− MF present on the extracellular side of the patch and the EC50 (black) and nH (blue) parameters. **, Ca2+ EC50 values are statistically different (P = 0.01) from the Ca2+ EC50 value calculated in 0 SCN− MF. The data were analyzed using one-way ANOVA with a Tukey’s post-hoc test. The gray curve shows the Ca2+ EC50 versus SCN− MF at +100 mV (n > 7). nH values were between 1.79 and 2.17.](https://cdn.rupress.org/rup/content_public/journal/jgp/154/8/10.1085_jgp.202113027/2/jgp_202113027_fig3.png?Expires=1771660953&Signature=qpBzGBRGFCRBkmtSBC3~bLr~ULbRZ0gPqaTDju1U50UaL7POtCfixmXTnFKnu6~bSCCeciTfd6EQbKa7DceUZMc07xRiSEsJ2yKcvhdm1CF0w2abL392yVROy5Xuwn4wRgPntDuLniXdYAt00sQkCoUbbXABxXaRXcjw97DX~U8pQlSCpf3d--oiW4dPX~7T3uOmv9Yw7E7JdQ16z479f6zpEBgecmhNB1qEu9JaQIIhUyEqM50dwzoMgR~G8d~Tz8~gTLJNroLgD~bPzjDTr1hhVV84WgAwhxqYvsfSYnp7kAeMBfTV54hn02SN3Z8ZxYhAoRu6L9If7INqCgZz3w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SCN − MFs induce AMF-like behavior on the apparent Ca 2+ sensitivity of WT TMEM16A. (A) Examples of macroscopic currents induced by applying increasing [Ca2+]i (indicated at the top of the panel) to the cytosolic side of four different inside-out patches held at +80 mV. The extracellular side of the patches was exposed to 0.25 (olive, n = 9), 0.5 (orange, n = 7), 0.75 (green, n = 5), or 1 (blue, n = 6) SCN− MF. [Cl−]i = 40 mM, pHe = pHi = 7.3. (B) Concentration–response curves constructed with the current magnitudes obtained with 0, 0.25, 0.5, 0.75, and 1 SCN− MF shown in A. Continuous lines are fits of Hill equation, used to determine EC50 and nH coefficient. (C) Relationship between SCN− MF present on the extracellular side of the patch and the EC50 (black) and nH (blue) parameters. **, Ca2+ EC50 values are statistically different (P = 0.01) from the Ca2+ EC50 value calculated in 0 SCN− MF. The data were analyzed using one-way ANOVA with a Tukey’s post-hoc test. The gray curve shows the Ca2+ EC50 versus SCN− MF at +100 mV (n > 7). nH values were between 1.79 and 2.17.