Enzymatic activities in the hearts of ELC mice. (A and B) Spectrophotometric assessment of enzymatic activities in heart homogenates from HCM-A57G (3 M, 3 F), Δ43 (3 M, 3 F), and RCM-E143K (4 M, 2 F) relative to WT (3 M, 3 F) mice (A), and separated by sex (B). Values are mean ± SD and analyzed using two-way ANOVA with Tukey’s or Sidak’s multiple comparisons test with significance depicted as &, P < 0.05 between HCM-A57G versus Δ43. Open symbols depict female mice and closed symbols depict male mice.