NPF interaction with EH ₂ domain in Eps15. (A) αB and αC helices (orange) and NPF motif (blue) from bound peptide PTGSSSTNPFR from the EH₂ domain of human EPS15 (PDB accession no. 1F8H). (B) Residues from the αB and αC helices that contact the NPF motif are indicated in grey boxes; interacting residues were determined in PyMOL as any contact <4 Å. Contacts between Asparagine of NPF and αB and αC helices are indicated by black lines; contacts between proline of NPF and αB and αC helices are indicated by purple lines; contacts between phenylalanine of NPF and αB and αC helices are indicated by green lines.