Phos-Tag phosphoprotein gel stain analysis to assess the phosphorylation status of myofilament proteins in WT and SA myocardial preparations. (A) Representative Phos-Tag phosphoprotein–stained (left) and Coomassie-stained (right) SDS gels showing the phosphorylation status of myofilament proteins in WT and SA samples before and following incubation with PKA. (B) Quantification of phosphorylation of cMyBPC, cTnT, cTnI, and RLC in WT and SA samples. In both groups, three hearts and two replicates each were used to analyze total protein expression and phosphorylation. Values are expressed as mean ± SEM. *, Significantly different compared with the corresponding pre-PKA group; P < 0.05.