Figure 5.
Transient adaptive responses in the postprandial python liver.(A and B) Western blot analysis reveals normalized MAPK stress kinase activation of ASK1, MKK3/6, and p38. (C) Western blot analysis indicates normalized AKT deactivation and FASN activation. (D) Immunohistochemical staining for BrdU incorporation into fasted (F) and 1DPF (1D) python livers. Scale bar, 50 µM. (E) Quantification of BrdU-positive cell numbers per stained fasted and 1DPF python liver sections. (F) qPCR for normalized Pcna (proliferating cell nuclear antigen) mRNA expression in fasted and 1, 3, and 10DPF python livers. Data are represented as mean ± SEM. n = 3/group. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001 versus fasted. Source data are available for this figure: SourceData F5.

Transient adaptive responses in the postprandial python liver. (A and B) Western blot analysis reveals normalized MAPK stress kinase activation of ASK1, MKK3/6, and p38. (C) Western blot analysis indicates normalized AKT deactivation and FASN activation. (D) Immunohistochemical staining for BrdU incorporation into fasted (F) and 1DPF (1D) python livers. Scale bar, 50 µM. (E) Quantification of BrdU-positive cell numbers per stained fasted and 1DPF python liver sections. (F) qPCR for normalized Pcna (proliferating cell nuclear antigen) mRNA expression in fasted and 1, 3, and 10DPF python livers. Data are represented as mean ± SEM. n = 3/group. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001 versus fasted. Source data are available for this figure: SourceData F5.

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