Emergence of distinguishing metabolite superpathways throughout digestion. (A and B) Heatmap displaying the rank-ordered levels of differentially regulated plasma: lipid (A) and amino acid (B) metabolites in fasted, 1DPF, and 3DPF Burmese pythons. The differences are log2 normalized and clustered by pattern of regulation between each state. For each state, n = 5/group. (C) PLS-DA of two component groups of plasma metabolites that are significantly different between feeding states in Burmese pythons. Each dot represents the metabolome of an individual animal. (D and E) Volcano plots for differentially expressed circulating metabolites at 1DPF (D) and 3DPF (E) versus fasted. The log₂ of the fold-change (FC) for each metabolite (gray dots) is on the x axis, and the −log₁₀ of the P value of this difference is on the y axis.