Postprandial hypertriglyceridemia. (A) Plasma NEFA levels, determined by colorimetric enzymatic assay. (B) Comparison of plasma TG in fasted/fed python, overnight fasted wild type mice at baseline and after a 0.75-ml bolus of corn oil delivered by oral gavage at 2.5, 7.5, and 24 h; and wild type, hypercholesterolemic Ldlr^−/− or leptin-deficient obese (ob/ob) Ldlr^−/− mice fasted for 6 h. (C and D) TG and cholesterol concentrations in plasma and isolated VLDL/chylomicron, LDL, and HDL fractions, determined by colorimetric enzymatic assays. (E) Coomassie-stained ApoB levels in the VLDL/chylomicron fraction of wild type C57BL/6 mice and pythons, determined by SDS-PAGE, or pre-stained molecular weight (MW) marker. (F) HDL phospholipid content in pythons, determined by colorimetric enzymatic assay. (G) Coomassie-stained HDL protein from fasted wild type, Ldlr, or ApoE knockout mice and fasted/fed pythons or MW marker. Data are represented as mean ± SD. n = 3/group. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 versus fasted (F) or WT. Source data are available for this figure: SourceData F1.