R92Q hiPSC-CMs show altered calcium transients and gene expression.(A) Representative fluorescence ratio traces showing calcium transients. Single hiPSC-CMs were seeded on rectangular patterns on 10 kPa hydrogels and loaded with the ratiometric calcium dye, Fura Red. R92Q hiPSC-CMs calcium transients have lower amplitudes than the WT cells. (B) Expression of key calcium-handling genes measured using qPCR. Data show significant increases in the expression of CASQ2, CAMK2D, and SLC8A1 and a decrease in CACNA1H. ΔCt values are shown in Table S2. Statistics were performed on the ΔCt values; however, we show the log-fold changes. Red lines show the means, boxes show the quartiles, and error bars show the standard deviations. Data shown reflect three biological replicates, each of which included three technical replicates. Asterisk denotes ΔCt values with P < 0.05 compared with the WT. P values were calculated using a Mann–Whitney test.
Figure 6.

R92Q hiPSC-CMs show altered calcium transients and gene expression. (A) Representative fluorescence ratio traces showing calcium transients. Single hiPSC-CMs were seeded on rectangular patterns on 10 kPa hydrogels and loaded with the ratiometric calcium dye, Fura Red. R92Q hiPSC-CMs calcium transients have lower amplitudes than the WT cells. (B) Expression of key calcium-handling genes measured using qPCR. Data show significant increases in the expression of CASQ2, CAMK2D, and SLC8A1 and a decrease in CACNA1H. ΔCt values are shown in Table S2. Statistics were performed on the ΔCt values; however, we show the log-fold changes. Red lines show the means, boxes show the quartiles, and error bars show the standard deviations. Data shown reflect three biological replicates, each of which included three technical replicates. Asterisk denotes ΔCt values with P < 0.05 compared with the WT. P values were calculated using a Mann–Whitney test.

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