Mechanosensitive channel traces of cells expressing pezo-1::GFP. Representative single-channel trace recordings of pressure-evoked currents from pezo-1::GFP cells expressing pezo-1 WT and R2373K, in the cell-attached configuration. Channel openings were elicited by −40 mmHg at a constant voltage of −60 mV. These are the same records shown in Fig. 7, A and B, used to generate the all-point amplitude histograms, filtered offline at 1 kHz to highlight single-channel events. Closed and open states are labeled c and o, respectively.