Figure 4. The 2R motif overrides the... | Rockefeller University Press

Figure 4.

The 2R motif overrides the effect from the S4/pore site. (A) Concentration-response curves for Wu50 at pH 9.0. 2R motif: EC50 = 30.0 ± 5.0 µM, ΔVMAX = −56.5 ± 2.6 mV, n = 4–10; 2R motif/W454A: EC50 = 27.9 ± 9.2 µM, ΔVMAX = −62.3 ± 6.5 mV, n = 3. Mean ± SEM. (B) 100 µM Wu50 at pH 9.0 shifts G(V) for the 2R motif/R362Q Shaker KV channel results by −59.0 mV. (C) Wu50-induced G(V) shifts for Shaker KV–channel mutants as indicated. 100 µM, pH = 9. Mean ± SEM (n = 4–10). **, P < 0.01. The red lines denote the shifts in the absence of the double arginine mutation (M356R/A359R = 2R).

The 2R motif overrides the effect from the S4/pore site. (A) Concentration-response curves for Wu50 at pH 9.0. 2R motif: EC₅₀ = 30.0 ± 5.0 µM, ΔV_MAX = −56.5 ± 2.6 mV, n = 4–10; 2R motif/W454A: EC₅₀ = 27.9 ± 9.2 µM, ΔV_MAX = −62.3 ± 6.5 mV, n = 3. Mean ± SEM. (B) 100 µM Wu50 at pH 9.0 shifts G(V) for the 2R motif/R362Q Shaker KV channel results by −59.0 mV. (C) Wu50-induced G(V) shifts for Shaker K_V–channel mutants as indicated. 100 µM, pH = 9. Mean ± SEM (n = 4–10). **, P < 0.01. The red lines denote the shifts in the absence of the double arginine mutation (M356R/A359R = 2R).

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