Time course of changes in LVP and average SL in an LV myocyte in a mouse under deep anesthesia. The mouse was anesthetized with ∼5% isoflurane. Black line indicates changes in the average length of nine sarcomeres in M1 (M1 Avg. SL) in Fig. 4 A, top left. Blue line indicates changes in LVP. In contrast to the mouse under normal physiological condition (ΔLVP 96.4 mm Hg), the LVP and average SL signals tended to be less coupled (compare Fig. 1 B).