Figure 6.
Figure 6. Sulfinamide formation on C36A-PLN at different concentrations of HNO donor. (a–d) Comparison of the sulfinamide signals generated on C36A-PLN (60 µM, Cys at positions 41 and 46) upon treatment with 1 mM (red) or 100 µM (blue) 15N-2-MSPA by 15N-edited 1H 2D-NMR (a) and 15N-edited 1H 1D-NMR analysis (b). Comparison of the sulfinamide signals generated on C46A-PLN (60 µM, Cys at positions 36 and 41) upon treatment with 1 mM (red) or 100 µM (blue) 15N-2-MSPA by 15N-edited 1H 2D-NMR (c) and 15N-edited 1H 1D-NMR analysis (d).

Sulfinamide formation on C36A-PLN at different concentrations of HNO donor. (a–d) Comparison of the sulfinamide signals generated on C36A-PLN (60 µM, Cys at positions 41 and 46) upon treatment with 1 mM (red) or 100 µM (blue) 15N-2-MSPA by 15N-edited 1H 2D-NMR (a) and 15N-edited 1H 1D-NMR analysis (b). Comparison of the sulfinamide signals generated on C46A-PLN (60 µM, Cys at positions 36 and 41) upon treatment with 1 mM (red) or 100 µM (blue) 15N-2-MSPA by 15N-edited 1H 2D-NMR (c) and 15N-edited 1H 1D-NMR analysis (d).

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