Western blotting analysis of APP processing and Aβ production in brain lysates of 4-mo-old, 6-mo-old, and 9-mo-old transgenic mice. (A–F) TBS-soluble, detergent-soluble, and FA-soluble fractions of brain lysates were subject to Western blot analysis, PS1ΔE9 animals were used as negative controls of exogenous APP expression, APP695Swe/S198P animals were used as negative controls of PS1ΔE9 expression, and ceAPPSwe/PS1ΔE9 animals were used as positive controls of APP processing and Aβ production. 4-mo-old animals (A and B), 6-mo-old animals (C and D), and 9-mo-old animals (E and F). Ab, antibody; FL, full-length.