Accuracy of parameters and equilibrium association constants. The optimal condition of including 16 CARs (CAR combination 95) was used. This CAR combination was fitted 1,000 times while stochastically varying the data points from fit to fit. The distribution of the accuracy, x_i/x_itr (Eq. 9), was evaluated. (A) Superimposed histograms of the distribution of accuracies for all 23 determined parameters. Bin width 0.01. The histograms are bell-shaped, differing significantly in their width. (B) Bar graph of the SDs obtained by fitting the histograms in A with a Gaussian function (Eq. 15). The SDs of all parameters are <0.1. (C) Superimposed histograms of the distribution of accuracies for the 32 equilibrium association constants K_i. Bin width 0.01. (D) Bar graph of the SDs obtained by fitting the histograms in C with a Gaussian function (Eq. 15). The SDs of the K_i were computed by Eqs. 2–4. The values are on average larger than the SDs of the Z_i (B) from which they were computed (see Materials and methods).