Mean uncertainty of the fits as function of the mutational effect. (A) Plot of three mean imprecisions, ipr_Ki,am, ipr_Ki,sm, and ipr_Ki,gm (Eqs. 6b, 7b, and 8b) corresponding to Fig. 7, A and B. (B) Plot of the three mean inaccuracies, iar_Ki,am, iar_Ki,sm, and iar_Ki,gm (Eqs. 11b, 12b, and 13b) corresponding to Fig. 7, A and B. The shaded areas illustrate that mutations decreasing the affinity by ≥60-fold generate properly determined parameters.