Effect of inclusion of different CAR numbers on Z _i , Ex, and K _i . Standard conditions. The abscissas indicate the number of the CAR combination according to Table 1. (A)Z_i and Ex as functions of the included CARs. The dimensions of Z₁–Z₄, Z₅–Z₁₀, Z₁₁–Z₁₄, and Z₁₅ are μM⁻¹, μM⁻², μM⁻³, and μM⁻⁴, respectively. The Ex are dimensionless. (B) σ_i,rel of Z_i and Ex as functions of the included CARs. Including more CARs decreases the imprecision of the fit. (C)K_i as function of the included CARs. The K_i were computed from the Z_i values according to the ratios specified in Table S2. (D) σ_i,rel of K_i as a function of the included CARs (see also Materials and methods).