The protein expression of cTn and tropomyosin was measured using quantitative immunoblotting (n = 8). (A) Representative bands are depicted by increasing the total amount of protein loading. (B) The signals of bands, measured and normalized to total protein signal as an internal loading control, are summarized. The expressions of troponin were lower in RVCM at the unsaturated ranges of protein loading, while that of tropomyosin was not different in all ranges.