The protein expression of SERCA and regulatory proteins. (A) The protein of two ventricles was extracted and blotted using SDS-PAGE. Representative blotting results are depicted. (B–D) Protein expression was normalized to GAPDH and summarized (n = 12). The amount of SERCA2a and PLB is not different in the two ventricles, while p-PLB is significantly lower in RVCM. (E) The ratio of p-PLB to PLB is also significantly lower in RVCM. All statistical tests performed using Student’s t test. ****, P < 0.0001.