Figure S2. Calibration factors for in... | Rockefeller University Press

Figure S2.

Calibration factors for in situ calibration of fura-2.(A) The constants of La3+ calibration were used for inhibiting hypercontraction. Gray areas represent SD. The fura-2 fluorescence intensity at 510 nm of emission wavelength according to the excitation wavelength (from 250 to 450 nm) in the cuvette-type spectrophotometer. The La3+-fura-2 spectra (black solid line) showed the higher fluorescence intensity at 340 nm, compared with that of 0.95 mM Ca2+ spectra (dotted solid line) had the lower fluorescence intensity at 380 nm. (B) Summary of the constants (C1 and C2) to correct the fluorescence intensity of La3+-fura-2 to Ca2+-fura-2 (see Materials and methods; n = 10).

Calibration factors for in situ calibration of fura-2. (A) The constants of La³⁺ calibration were used for inhibiting hypercontraction. Gray areas represent SD. The fura-2 fluorescence intensity at 510 nm of emission wavelength according to the excitation wavelength (from 250 to 450 nm) in the cuvette-type spectrophotometer. The La³⁺-fura-2 spectra (black solid line) showed the higher fluorescence intensity at 340 nm, compared with that of 0.95 mM Ca²⁺ spectra (dotted solid line) had the lower fluorescence intensity at 380 nm. (B) Summary of the constants (C1 and C2) to correct the fluorescence intensity of La³⁺-fura-2 to Ca²⁺-fura-2 (see Materials and methods; n = 10).