Figure 1. CD spectroscopy . MelB St... | Rockefeller University Press

Figure 1.

$CD spectroscopy. MelBSt samples at a protein concentration of 10 µM in the CD assay buffer (10 mM KPi, pH 7.5, 10% glycerol, and 0.035% UDM) in the absence or presence of a salt and/or melibiose at 50 mM were prepared for CD measurements as described in Materials and methods. Left: Far-UV CD spectra. The presented spectra were recorded between 200 and 260 nm at 25°C after subtracting from each the corresponding buffer control. mdeg, millidegree. Right: Thermal denaturation. The CD ellipticity changes were recorded at 210 nm at a 1°C interval, with the temperature ramping up at 1°C per minute, and are expressed as a percentage of the unfolded fraction. (a) WT MelBSt. (b) D55C MelBSt mutant. The color legends indicate the assay conditions.$

CD spectroscopy . MelB_St samples at a protein concentration of 10 µM in the CD assay buffer (10 mM KPi, pH 7.5, 10% glycerol, and 0.035% UDM) in the absence or presence of a salt and/or melibiose at 50 mM were prepared for CD measurements as described in Materials and methods. Left: Far-UV CD spectra. The presented spectra were recorded between 200 and 260 nm at 25°C after subtracting from each the corresponding buffer control. mdeg, millidegree. Right: Thermal denaturation. The CD ellipticity changes were recorded at 210 nm at a 1°C interval, with the temperature ramping up at 1°C per minute, and are expressed as a percentage of the unfolded fraction. (a) WT MelB_St. (b) D55C MelB_St mutant. The color legends indicate the assay conditions.