Oleoyl-CoA regulation of K_2P channels. (A–H) Representative current traces (right) and analyzed currents at +40 mV plotted over time (left) of oleoyl-CoA–activated (light blue) TREK-1 (A), TREK-2 (B), TALK-1 (C), TASK-1 (D), THIK-1 (F), and THIK-2* (G) and oleoyl-CoA–inhibited (brown) TASK-3 (E) and TRESK (H) K_2P channels. Currents were measured in voltage ramps between −80 and +80 mV in excised inside-out patches of Xenopus oocytes using K⁺ or Rb⁺ (red) bath solutions and in the presence of 10 µM PIP₂ or 5 mg ml⁻¹ BSA (pink) added to the standard K⁺ solution.