Representative traces of the PIP₂ regulation of K_2P channels and PLC-mediated inhibition of TREK-1 channels. (A–J) Representative current traces (right) and analyzed currents at +40 mV plotted over time (left) of PIP₂-activated (blue) TREK-1 (A), TREK-2 (B), TRAAK (C), TALK-1 (D), TALK-2 (E), THIK-2*- and PIP₂-inhibited (orange) TASK-3 (G), and TASK-2 K245A (J) K_2P channels. TWIK-1* (F) and TRESK (I) are not affected by PIP₂. Currents were measured in voltage ramps between −80 and +80 mV in excised inside-out patches of Xenopus oocytes using K⁺ or Rb⁺ (red) bath solutions and in the presence of 10 µM PIP₂ or DMSO (1%) added to the standard K⁺ solution. (K) Representative traces of TREK-1 currents in whole-cell experiments using HEK293 cells measured in voltage ramps between −100 and +60 mV and analyzed at 0 mV. Measurements were performed in control solution and in the presence of 20 µM of the PLC activator m-3M3FBS. Additionally, the temperature was increased to 37°C (red) and subsequently decreased (blue) to room temperature of 21°C (blue).