Histological sections, morphometry, and weight of E18.5 urinary bladders. (A and B) Representative images of WT (A) and KO (B) of Masson-Goldner–stained 7.5-µm sagittal sections of whole embryo mounts at three different magnifications, showing the urinary bladder with the urothel, the lamina propria, and the tunica muscularis. Scale bars, which are incorporated in the figures, are also shown below the sections for better visibility. (C) In each fetus (n = 4 per genotype), three adjacent sections were analyzed and averaged; symbols represent the averaged values per fetus. Bars: mean ± SD; no significant difference was observed. (D and E) Bladder weight (D) and ratio of urinary bladder weight (UBW) to body weight (BW; E) was not different between genotypes. Symbols and bars represent mean ± SD.