Localization of TRPM7 or its mutants in HEK293 cells. Representative confocal images showing the localization of TRPM7 (green) in doxycycline-untreated (Dox[−]) and mTRPM7-wt (WT)–, -C721A–, -C738A–, -C721S–, -C738S–, -C1809S–, and -C1813S–expressing HEK293 cells. Phase-contrast images are also shown. Scale bar, 20 µm.