Figure 2.
![Screening of cysteines in mTRPM7 that were responsible for the oxidative stress–induced inhibition.(A) Schematic diagram of 34 cysteines that are conserved in mTRPM7 and hTRPM7. S1107 and D1510 are also depicted (red dots). (B) Expression of TRPM7-wt and its mutants. Immunoblotting of TRPM7 and calnexin (loading control) was performed with HEK293 whole-cell lysates. Numbers on the left represent molecular mass of standards (in kD). (C) The effect of H2O2 (500 µM) on mutant TRPM7 current at 0.2 mM [Mg2+]i. Each bar represents the mean ± SEM (vertical bar). *, P < 0.05 by unpaired t test versus Dox[−] cells. The number of observations (n) and exact P values for each mutant are shown in Table S1.](https://cdn.rupress.org/rup/content_public/journal/jgp/153/6/10.1085_jgp.202012708/2/jgp_202012708_fig2.png?Expires=1767702996&Signature=3Y0m7ogYo4prDKhYl~gLiP75ZYxGWEYUFRt25q7TP4qHX6iDl1hoDlMtwVpcHVYwA1WrTL1zurRBVhcytgSUquwLQu~Ry~D4GnOAlE7NdueQ7b2dFTxWxzilLbG6oXc1LwYO25xhfnBp9zhkVUHjvl0R33Valu17AeWq5tB6YQpBwArbsrEUt1C21Ar7sksMoFRpN~FJVKdw~WLsf-27XBMjVG9RIXXJW4MisXijDE1ltI4bR11li3c6UWRmjwK6-qC-4qmd8vbw-bj6xcOndjG56p-vfwZjNfDRCiIgu6sMJor9PycdhVr2sgV32-h4ySG-tz-P3pUcBgRfpZzTog__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Screening of cysteines in mTRPM7 that were responsible for the oxidative stress–induced inhibition. (A) Schematic diagram of 34 cysteines that are conserved in mTRPM7 and hTRPM7. S1107 and D1510 are also depicted (red dots). (B) Expression of TRPM7-wt and its mutants. Immunoblotting of TRPM7 and calnexin (loading control) was performed with HEK293 whole-cell lysates. Numbers on the left represent molecular mass of standards (in kD). (C) The effect of H2O2 (500 µM) on mutant TRPM7 current at 0.2 mM [Mg2+]i. Each bar represents the mean ± SEM (vertical bar). *, P < 0.05 by unpaired t test versus Dox[−] cells. The number of observations (n) and exact P values for each mutant are shown in Table S1.