Figure S1.

The I131G mutant is more permeable to protons. (A) Schematic diagram of K-driven 86Rb+ assays with external pH values varying from 7 to 9. (B) Relative counts of 86Rb+ uptake after 10 min for KirBac1.1 WT (gray bars) and KirBac1.1 I131G (blue bars) with increasing pH (7–9), normalized to counts at pH 7. All data are represented as mean ± SEM of at least n = 3 experiments.

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