Cosedimentation assays for C0–C1 binding to actin–Tm and actin. C0–C1 (red lines) was assayed and unphosphorylated C0–C2 (solid lines), and phosphorylated C0–C2 (dotted lines) are shown for reference. (A) Cosedimentation using actin–Tm. (B) Cosedimentation using actin. Cosedimentation using actin–Tm or actin was also measured at 40 µM C0–C1, and this was used for determination of K_d and B_max for C0–C1 curves (Table S1). Data are provided as mean ± SE (n > 4).