Figure 3.
Cy3B-telenzepine–labeled M2 muscarinic receptors on the plasma membrane of mouse cardiomyocytes.(A) Left: A single video frame image of a primary-cultured cardiomyocyte, viewed by TIRFM. M2 receptors labeled with Cy3-telenzepine are visible as individual spots. Right: The same cell is shown as a normalized SD z-projection created from ∼500 video frames. (B) Left: A single video frame image of a small region of mouse cardiac tissue. Again, M2 receptors are visualized as individual spots of light. Right: The same cell is shown as a normalized SD z-projection from ∼500 video frames. (C and D) Cartoons depicting the TIRF imaging conditions in A and B. (E) Enlarged boxed region from A showing individual fluorophore trajectories (red traces). (F) The averaged MSD plotted against dT for M2 receptor movements in cardiac tissue slices compared with isolated cardiomyocytes. In this experiment, initial gradients are 2.56 and 0.79 µm2 ⋅ s−1, giving Dlat = 0.64 and 0.2 µm2 ⋅ s−1, respectively. See Table 1. (G) Histograms showing the distribution of Dlat estimates made for thousands of individual molecular trajectories measured from the two preparations.

Cy3B-telenzepine–labeled M2 muscarinic receptors on the plasma membrane of mouse cardiomyocytes. (A) Left: A single video frame image of a primary-cultured cardiomyocyte, viewed by TIRFM. M2 receptors labeled with Cy3-telenzepine are visible as individual spots. Right: The same cell is shown as a normalized SD z-projection created from ∼500 video frames. (B) Left: A single video frame image of a small region of mouse cardiac tissue. Again, M2 receptors are visualized as individual spots of light. Right: The same cell is shown as a normalized SD z-projection from ∼500 video frames. (C and D) Cartoons depicting the TIRF imaging conditions in A and B. (E) Enlarged boxed region from A showing individual fluorophore trajectories (red traces). (F) The averaged MSD plotted against dT for M2 receptor movements in cardiac tissue slices compared with isolated cardiomyocytes. In this experiment, initial gradients are 2.56 and 0.79 µm2 ⋅ s−1, giving Dlat = 0.64 and 0.2 µm2 ⋅ s−1, respectively. See Table 1. (G) Histograms showing the distribution of Dlat estimates made for thousands of individual molecular trajectories measured from the two preparations.

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