Figure S5.
Panx1 colocalizes with Piezo1 in cholangiocyte plasma membrane.(A) RT-PCR for Panx1 in DIV5 cholangiocytes and NMCs. Black dots indicate the 500-bp DNA marker. (B) Anti-flag and anti-Panx1 immunostainings in DIV5 cholangiocytes (bottom) and in HEK293T-P1KO cells transfected with flag-panx1 cDNA (top). (C) Western blot analysis of Panx1 expression in whole cell lysates from HEK293T-P1KO cells expressing Panx1-flag (left), cholangiocytes (middle), and NMCs (right; see also Fig. 7). (D) Immunofluorescent staining of Piezo1-GFP and Pannexin1-flag overexpressed in HEK293T-P1KO cells. Cells were labeled using an Alexa 647–coupled anti-flag (shown in green) and a TRITC (tetramethylrhodamine)-coupled anti-GFP (shown in red). (E) The line scan illustrates overlap of Piezo1-GFP and Panx1-flag labels at the cell periphery.

Panx1 colocalizes with Piezo1 in cholangiocyte plasma membrane. (A) RT-PCR for Panx1 in DIV5 cholangiocytes and NMCs. Black dots indicate the 500-bp DNA marker. (B) Anti-flag and anti-Panx1 immunostainings in DIV5 cholangiocytes (bottom) and in HEK293T-P1KO cells transfected with flag-panx1 cDNA (top). (C) Western blot analysis of Panx1 expression in whole cell lysates from HEK293T-P1KO cells expressing Panx1-flag (left), cholangiocytes (middle), and NMCs (right; see also Fig. 7). (D) Immunofluorescent staining of Piezo1-GFP and Pannexin1-flag overexpressed in HEK293T-P1KO cells. Cells were labeled using an Alexa 647–coupled anti-flag (shown in green) and a TRITC (tetramethylrhodamine)-coupled anti-GFP (shown in red). (E) The line scan illustrates overlap of Piezo1-GFP and Panx1-flag labels at the cell periphery.

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