Simulated Zn²⁺ effects on Ca_v2.3 channel voltage dependence. (A–C) Families of simulated current traces evoked with the protocols depicted above (for details, see Fig. 2), with the free Zn²⁺ concentration set to 0 (black) or 5.4 µM (orange). Scale bar corresponds to 5 ms and 20 pA/pF in A, 5 ms and 50 pA/pF in B, or 10 ms and 200 pA/pF in C. (D) I-V relationships determined from the simulated currents shown in A. (E) II-V relationships determined from the simulated currents shown in C. (F) Isochronous activation (right) and PPI (left) curves determined from the simulated tail currents in A or the simulated peak currents in B, respectively. (G) Concentration dependence of simulated Zn²⁺-induced suppression, quantified in terms of changes in G_max of simulated currents observed when the free Zn²⁺ concentration was increased from 0 to 1,000 µM.