Figure 7.
The K1493E/E1784K double mutant abolishes noninactivating current, but not the changes to fast inactivation voltage dependence or rates.(A) Sample currents recorded at −20 mV from E1784E (black), K1493E (blue), E1784K (red), K1493E/E1784K (purple), and K1641D (orange) channels at extracellular pH 7.4 normalized to the peak inward current. Sample currents shown were from cells with similar peak sodium currents. (B) Average noninactivating current as a percent of peak current for E1784E (EE), K1493E (KE), E1784K (EK), K1492E/E1784K (1492), K1500E/E1784K (1500), K1493E/E1784K (KEEK), and K1641D (KD) channels recorded at −20 mV at pH 7.4 (n = 3–7; Table S8). (C) Average voltage dependence of fast inactivation in E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D channels at extracellular pH 7.4. (D) Average midpoints of the fast inactivation voltage dependence for E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D at pH 7.4 (n = 5–9; Table S2). (E) Average time course of fast inactivation recovery at −90 mV in E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D channels at extracellular pH 7.4 (n = 3–7; Table S3). (F) Average time course of fast inactivation onset at −50 mV in E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D channels at extracellular pH 7.4 (n = 3–7; Table S4). Error bars are SEM.

The K1493E/E1784K double mutant abolishes noninactivating current, but not the changes to fast inactivation voltage dependence or rates. (A) Sample currents recorded at −20 mV from E1784E (black), K1493E (blue), E1784K (red), K1493E/E1784K (purple), and K1641D (orange) channels at extracellular pH 7.4 normalized to the peak inward current. Sample currents shown were from cells with similar peak sodium currents. (B) Average noninactivating current as a percent of peak current for E1784E (EE), K1493E (KE), E1784K (EK), K1492E/E1784K (1492), K1500E/E1784K (1500), K1493E/E1784K (KEEK), and K1641D (KD) channels recorded at −20 mV at pH 7.4 (n = 3–7; Table S8). (C) Average voltage dependence of fast inactivation in E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D channels at extracellular pH 7.4. (D) Average midpoints of the fast inactivation voltage dependence for E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D at pH 7.4 (n = 5–9; Table S2). (E) Average time course of fast inactivation recovery at −90 mV in E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D channels at extracellular pH 7.4 (n = 3–7; Table S3). (F) Average time course of fast inactivation onset at −50 mV in E1784E, K1493E, E1784K, K1493E/E1784K, and K1641D channels at extracellular pH 7.4 (n = 3–7; Table S4). Error bars are SEM.

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