Figure 1.
![MthK channel blockade by SPD3+.(A) Mean normalized macroscopic MthK currents. Currents were obtained over a range of [SPD3+] (≤1,000 µM) using a voltage-ramp protocol and normalized to the current amplitude at −100 mV for the same bilayer with 0 SPD3+. (B) Fractional current versus [SPD3+] plotted at five representative voltages. Data were fitted with Hill equations (smooth curves) to estimate Kapp over a range of voltages (parameters are in Table S1). (C)Kapp versus voltage, fitted with Eq. 1 (solid blue line; Kapp(0) = 860 ± 20 µM, zδ = 0.67 ± 0.03 e0).](https://cdn.rupress.org/rup/content_public/journal/jgp/152/7/10.1085_jgp.201912527/3/jgp_201912527_fig1.png?Expires=1767736008&Signature=X9viQuZGgLGzNRIcmWccrdZQJagm8~JH85YeaxHYTts6JlCaxcrfNYKMfYmoCQc2GxBQvwOgw~6DT6ZYgoSN~qhgSlGLjQndakIJJEovq1aCeYhrtTmJjN4H7wdoZteg7R-V~ypn0PJntjB-kyhfXTk00i~ZG-L8QHMdSZJAf--lOPCyGHpomJyWmr2KRg0syPQ-0QieaOmhF6jSCKpnhF0ixb9qZAwU6nniwDPp-wUYudTqyx33SpEAtFfvH5gvbQfBlzJkX7FwGU8nUF74zLkMkVZqI4sEmmxDjOmq3FQhcVXksnD45evOfnwrgQLx0RvG5llGXcrQ0LgWav7TdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MthK channel blockade by SPD3+. (A) Mean normalized macroscopic MthK currents. Currents were obtained over a range of [SPD3+] (≤1,000 µM) using a voltage-ramp protocol and normalized to the current amplitude at −100 mV for the same bilayer with 0 SPD3+. (B) Fractional current versus [SPD3+] plotted at five representative voltages. Data were fitted with Hill equations (smooth curves) to estimate Kapp over a range of voltages (parameters are in Table S1). (C)Kapp versus voltage, fitted with Eq. 1 (solid blue line; Kapp(0) = 860 ± 20 µM, zδ = 0.67 ± 0.03 e0).