Figure S4.
Stability of Orai1-GCaMP6f resting fluorescence and relative STIM1/Orai1 ratio.(A) Plots of Orai1-GCaMP6f resting fluorescence from each cell footprint, normalized to the average baseline value before four test pulses (−10, −40, −70, and −100 mV in 2 Ca). SD was 1.6% (24 total measurements). (B) Plots of Orai1-GCaMP6f resting fluorescence of 112 individual puncta from cell A (see Fig. S3). Note consistency in resting fluorescence for individual puncta. (C) Plots of normalized Orai1-GCaMP6f resting fluorescence from 535 individual puncta from cells A–F. (D) Plots of normalized relative STIM1/Orai1 ratio from 535 puncta from cells A–F. SDs of C and D were 3.6% and 3.8%, respectively (2,140 measurements each). Red lines in C and D are mean and SD. Relative STIM1/Orai1 ratio decreased by 0.7% between the first (−10 mV) and last (−100 mV) test pulses.

Stability of Orai1-GCaMP6f resting fluorescence and relative STIM1/Orai1 ratio. (A) Plots of Orai1-GCaMP6f resting fluorescence from each cell footprint, normalized to the average baseline value before four test pulses (−10, −40, −70, and −100 mV in 2 Ca). SD was 1.6% (24 total measurements). (B) Plots of Orai1-GCaMP6f resting fluorescence of 112 individual puncta from cell A (see Fig. S3). Note consistency in resting fluorescence for individual puncta. (C) Plots of normalized Orai1-GCaMP6f resting fluorescence from 535 individual puncta from cells A–F. (D) Plots of normalized relative STIM1/Orai1 ratio from 535 puncta from cells A–F. SDs of C and D were 3.6% and 3.8%, respectively (2,140 measurements each). Red lines in C and D are mean and SD. Relative STIM1/Orai1 ratio decreased by 0.7% between the first (−10 mV) and last (−100 mV) test pulses.

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