Figure 9.
Regulation of Kv1.2[L298F] by Kvβ. (A) LM cells were transfected with Kv1.2[L298F] in the absence or presence of Kvβ. Left: Representative current traces of the activation curves, with the pulse to −20 mV highlighted in green. Right: G–V relationships were measured as described in Fig. 3 A. G–V relationships were generated from the tail current amplitudes and fitted with a Boltzmann function. Kv1.2[L298F] V1/2 = −60 ± 5 (mean ± SD), k = 7.2 ± 1.6, n = 10 individual cells recorded; Kv1.2[L298F] + Kvβ V1/2 = −66.6 ± 2.3, k= 4.1 ± 0.6, n = 8. (B)V1/2 of individuals cells from Kv1.2[L298F] and Kv1.2[L298F] + Kvβ (green) along with select V1/2 values from Fig. 1 and Fig. S1 (gray). (C) Overlapping deactivation tail currents of Kv1.2[L298F] and Kv1.2[L298F] + Kvβ at −130 mV from a +100-mV depolarizing voltage. Deactivation tails of Kv1.2[L298F] and Kv1.2[L298F] + Kvβ were fitted with a single exponential function. (D) Time constants of deactivation (τ deact at −130 mV) calculated from single exponential functions of the deactivation tail currents of Kv1.2[L298F] and Kv1.2[L298F] + Kvβ obtained using the voltage protocol described in Fig. 7 A (mean ± SD; Kv1.2[L298F] Ctrl = 44 ± 13 ms, Kv1.2[L298F] + Kvβ = 98 ± 18 ms; n = 12–13 individual cells recorded for each group). (E) Steady-state inactivation plots of Kv1.2[L298F] + Kvβ were generated using the voltage protocol described in Fig. 4 A (n = 7 individual cells recorded). Data from Fig. 4 D are superimposed for comparison. (F) Time constants of inactivation (at +100 mV) were calculated using the voltage protocol described in Fig. 5 A (mean ± SD; Kv1.2[L298F] + Kvβ = 8.0 ± 2.8 ms; n = 19 individual cells recorded). Data from Fig. 5 A are superimposed for comparison. (G) Recovery from inactivation was measured using the voltage protocol described in Fig. 6 A (n = 11 individual cells recorded). Data from Fig. 6 A are superimposed for comparison (black dotted lines). Box plots depict the median, 25th, and 75th percentiles (box) and the 10th and 90th percentiles (whiskers).

Regulation of Kv1.2[L298F] by Kvβ. (A) LM cells were transfected with Kv1.2[L298F] in the absence or presence of Kvβ. Left: Representative current traces of the activation curves, with the pulse to −20 mV highlighted in green. Right: G–V relationships were measured as described in Fig. 3 A. G–V relationships were generated from the tail current amplitudes and fitted with a Boltzmann function. Kv1.2[L298F] V1/2 = −60 ± 5 (mean ± SD), k = 7.2 ± 1.6, n = 10 individual cells recorded; Kv1.2[L298F] + Kvβ V1/2 = −66.6 ± 2.3, k= 4.1 ± 0.6, n = 8. (B)V1/2 of individuals cells from Kv1.2[L298F] and Kv1.2[L298F] + Kvβ (green) along with select V1/2 values from Fig. 1 and Fig. S1 (gray). (C) Overlapping deactivation tail currents of Kv1.2[L298F] and Kv1.2[L298F] + Kvβ at −130 mV from a +100-mV depolarizing voltage. Deactivation tails of Kv1.2[L298F] and Kv1.2[L298F] + Kvβ were fitted with a single exponential function. (D) Time constants of deactivation (τ deact at −130 mV) calculated from single exponential functions of the deactivation tail currents of Kv1.2[L298F] and Kv1.2[L298F] + Kvβ obtained using the voltage protocol described in Fig. 7 A (mean ± SD; Kv1.2[L298F] Ctrl = 44 ± 13 ms, Kv1.2[L298F] + Kvβ = 98 ± 18 ms; n = 12–13 individual cells recorded for each group). (E) Steady-state inactivation plots of Kv1.2[L298F] + Kvβ were generated using the voltage protocol described in Fig. 4 A (n = 7 individual cells recorded). Data from Fig. 4 D are superimposed for comparison. (F) Time constants of inactivation (at +100 mV) were calculated using the voltage protocol described in Fig. 5 A (mean ± SD; Kv1.2[L298F] + Kvβ = 8.0 ± 2.8 ms; n = 19 individual cells recorded). Data from Fig. 5 A are superimposed for comparison. (G) Recovery from inactivation was measured using the voltage protocol described in Fig. 6 A (n = 11 individual cells recorded). Data from Fig. 6 A are superimposed for comparison (black dotted lines). Box plots depict the median, 25th, and 75th percentiles (box) and the 10th and 90th percentiles (whiskers).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal