Slc7a5 alters steady-state inactivation of Kvβ-regulated Kv1.2 channels. LM cells were transfected with Kv1.2[T252R] + Kvβ; or Kv1.2[T252R] + Kvβ + Slc7a5 with transfection ratios as previously mentioned. Cells were hyperpolarized to −120 mV for 30 s before recording, to disinhibit Kv1.2 currents. (A) Representative current traces of Kv1.2[T252R] + Kvβ and Kv1.2[T252R] + Kvβ + Slc7a5 used to measure the steady-state inactivation plots in B. Magnified insets highlight the test pulse at +30 mV from prepulse traces at +30, −30, −90, and −130 mV. (B) Steady-state inactivation of Kv1.2[T252R] + Kvβ and Kv1.2[T252R] + Kvβ + Slc7a5 were measured by stepping from +50 to −130 mV in 10-mV increments for 1 s followed by a test pulse to +30 mV for 100 ms. Steady-state inactivation plots (mean values ± SEM) were generated by analyzing current amplitudes at the +30-mV test pulse from each prepulse voltage (n = 5 individual cells recorded for each group). Box plots depict the median, 25th, and 75th percentiles (box) and the 10th and 90th percentiles (whiskers).