Comparison of membrane fluorescence and photocurrents in Xenopus oocytes expressing ChR2-YFP and β1-ChR2-YFP. (A) Representative fluorescence images of Xenopus oocytes. Noninjected control oocytes showed only faint background fluorescence. Scale bars: 0.25 mm. (B) Normalized fluorescence intensities. Data points are from three different oocyte batches with n = 22 for ChR2-YFP and n = 19 for β1-ChR2-YFP (*, P < 0.05 versus ChR2-YFP). (C) Representative whole-cell currents through ChR2-YFP and β1-ChR2-YFP. (D) Peak current amplitudes. Number of measurements were n = 27 for ChR2-YFP and n = 26 for β1-ChR2-YFP (*, P < 0.05 versus ChR2-YFP). Four different oocyte batches were used. Error bars represent SEM.