Figure 2.
Electrophysiological test for functional expression of ChR-Nav1.5 and Nav1.5-ChR2 in Xenopus oocytes.(A) Representative whole-cell currents generated by wild type Nav1.5, ChR2-Nav1.5, and Nav1.5-ChR2 at test potentials between −45 and +20 mV. Injected amount of cRNA per oocyte: 0.1 ng Nav1.5, 10 ng ChR2-Nav1.5, and 10 ng Nav1.5-ChR2. Peak current density for Nav1.5 was 1.56 ± 0.1 µA (n = 10). Current reduction was similar in ChR2-Nav1.5 and Nav1.5-ChR2 (0.59 ± 0.08 µA with n = 21 versus 0.71 ± 0.07 µA with n = 27, respectively; P < 0.05 versus Nav1.5). (B) None of the ChR2 fusions responded to a 1-s blue-light pulse. Injected amount of cRNA per oocyte: 0.2 ng ChR2, 10 ng ChR2-Nav1.5, and 10 ng Nav1.5-ChR2.

Electrophysiological test for functional expression of ChR-Nav1.5 and Nav1.5-ChR2 in Xenopus oocytes. (A) Representative whole-cell currents generated by wild type Nav1.5, ChR2-Nav1.5, and Nav1.5-ChR2 at test potentials between −45 and +20 mV. Injected amount of cRNA per oocyte: 0.1 ng Nav1.5, 10 ng ChR2-Nav1.5, and 10 ng Nav1.5-ChR2. Peak current density for Nav1.5 was 1.56 ± 0.1 µA (n = 10). Current reduction was similar in ChR2-Nav1.5 and Nav1.5-ChR2 (0.59 ± 0.08 µA with n = 21 versus 0.71 ± 0.07 µA with n = 27, respectively; P < 0.05 versus Nav1.5). (B) None of the ChR2 fusions responded to a 1-s blue-light pulse. Injected amount of cRNA per oocyte: 0.2 ng ChR2, 10 ng ChR2-Nav1.5, and 10 ng Nav1.5-ChR2.

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