Changes in WGA-pHRho fluorescence requires exogenous expression of proton channels in CHO cells. Related to Fig. 2.(A and B) Current–voltage relationships for (A) Hv-1 and (B) Shaker-IR (R362H/W434F) expressed in CHO cells. Hv-1 was held at −80 mV, and currents were elicited from 4-s command voltages from 0 to 100 mV in 20-mV increments; Shaker-IR (R362H/W434F) was held at 30 mV, and the currents were elicited from 4-s command voltages from −120 to −20 mV in 20-mV increments. (C and D) Voltage-clamp fluorometry of CHO cells transfected with either (C) pCDNA3.1(−) or (D) WT Shaker-IR controls. The cells were held at −80 mV and depolarized to for 4 s; pCDNA3.1(−), 100 mV; WT Shaker-IR, 20 mV. An outward pH gradient (pHo/pHi = 7.5/6.0) was used in A and C, and an inward pH gradient (pHo/pHi = 6.0/7.5) was used in B and D. The buffer (HEPES) capacity was 0.1 mM for all experiments.
Figure S2.

Changes in WGA-pHRho fluorescence requires exogenous expression of proton channels in CHO cells. Related to Fig. 2 . (A and B) Current–voltage relationships for (A) Hv-1 and (B) Shaker-IR (R362H/W434F) expressed in CHO cells. Hv-1 was held at −80 mV, and currents were elicited from 4-s command voltages from 0 to 100 mV in 20-mV increments; Shaker-IR (R362H/W434F) was held at 30 mV, and the currents were elicited from 4-s command voltages from −120 to −20 mV in 20-mV increments. (C and D) Voltage-clamp fluorometry of CHO cells transfected with either (C) pCDNA3.1(−) or (D) WT Shaker-IR controls. The cells were held at −80 mV and depolarized to for 4 s; pCDNA3.1(−), 100 mV; WT Shaker-IR, 20 mV. An outward pH gradient (pHo/pHi = 7.5/6.0) was used in A and C, and an inward pH gradient (pHo/pHi = 6.0/7.5) was used in B and D. The buffer (HEPES) capacity was 0.1 mM for all experiments.

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