Figure 2.
The sole expression of D1R increases CaV2.2 currents in transfected HEK293T cells. (A) Photomicrographs and average YFP fluorescence (D1R-YFP F) in the plasma membrane (PM) normalized by cell area in HEK293T cells cotransfected with CaV2.2, CaVα2δ1, CaVβ3, and two different amounts of D1R-YFP (11 and 23 ng cDNA per well, equal to 0.05 and 0.1 D1R:CaV2.2 molar ratios, respectively). Green and red signals originate from the YFP tag on D1R and the PM marker CellMask (PM marker), respectively. Scale bar represents 1 µm. (B) Representative traces and averaged CaV2.2 currents (ICav2.2) from HEK293T cells cotransfected with CaV2.2, CaVα2δ1, CaVβ3, and increasing amounts of D1R (0–23 ng cDNA per well). Student’s unpaired t test (A) and Kruskal-Wallis test with Dunn’s post-test versus 0 ng D1R (B). n.s., nonstatistically significant. Data were expressed as mean ± SEM, and dots represent individual data points.

The sole expression of D1R increases CaV2.2 currents in transfected HEK293T cells. (A) Photomicrographs and average YFP fluorescence (D1R-YFP F) in the plasma membrane (PM) normalized by cell area in HEK293T cells cotransfected with CaV2.2, CaVα2δ1, CaVβ3, and two different amounts of D1R-YFP (11 and 23 ng cDNA per well, equal to 0.05 and 0.1 D1R:CaV2.2 molar ratios, respectively). Green and red signals originate from the YFP tag on D1R and the PM marker CellMask (PM marker), respectively. Scale bar represents 1 µm. (B) Representative traces and averaged CaV2.2 currents (ICav2.2) from HEK293T cells cotransfected with CaV2.2, CaVα2δ1, CaVβ3, and increasing amounts of D1R (0–23 ng cDNA per well). Student’s unpaired t test (A) and Kruskal-Wallis test with Dunn’s post-test versus 0 ng D1R (B). n.s., nonstatistically significant. Data were expressed as mean ± SEM, and dots represent individual data points.

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