CaV currents from mPFC neurons are sensitive to D1/D5R constitutive and agonist-evoked activity. (A) Schematic coronal slice of a mouse brain containing the mPFC (1.75 mm anterior to bregma), showing the location of the patch pipette in layer V of the mPFC (dark gray area). Inset shows an infrared differential interference contrast image illustrating a recorded layer V/VI pyramidal neuron. Scale bar represents 10 µm. (B) Representative time courses and averaged values of percentage of CaV current (ICa) inhibition from mPFC neurons by dopamine (+DA; 10 µM; n = 4, three animals) and SKF38393 (+SKF; 10 µM; n = 5, three animals). (C) Representative time course of ICa and averaged values for the percentage of ICa inhibition from mPFC neurons by subsequent application of quinpirole (QP; 40 µM) and dopamine (+DA; 10 µM; n = 3, two animals). (D) Representative traces and averaged normalized ICa registered in mPFC of mice treated IP with vehicle (n = 23, eight animals) or chlorpromazine (CPZ; 1 mg/kg; n = 6, four animals). (E) Representative time courses of ICa and averaged percentage of inhibition of ICa by acute application of ω-conotoxin GVIA (cono; 1 µM) from mPFC neurons of mice treated IP with vehicle (n = 10, six animals) or CPZ (1 mg/kg; n = 5, four animals). (F) Representative time course of ICa and averaged values for the percentage of inhibition of ICa from mPFC neurons by subsequent application of 10 µM CPZ and 10 µM dopamine (+DA; n = 4, three animals). Student’s unpaired (B, D, and E) and paired (C and F) t test. n.s., nonstatistically significant. Data were expressed as mean ± SEM, and dots represent individual data points.