Schematic diagram of the movements of each domain in the WT and hypothetical movements of each domain in the R402C/H mutants. Each domain in the closed state is color coded: NTD is shown in light blue; Handle-HD1-P2-HD2 is shown in yellow-green; SPRY1-P1-SPRY2-SPRY3 is shown in light green; Central is shown in orange; and S6, the channel pore, is shown in red. Left: The movements of each domain in the WT. The place of each domain in the open state is shown transparently. Red arrows indicate the actual movements of the domains observed in the cryo-EM structures. Right: A hypothetical movement of each domain in the R402C/H mutants. The place of each domain in the mutants after 50 ns of the MD simulation is shown transparently. Blue arrows indicate the hypothetical movements.