Western blot analyses of proteins that coordinate SOCE and SR Ca²⁺ uptake. (A–C) Representative immunoblots showing expression levels of the long (STIM1L) and short (STIM1S) STIM1 isoforms (A), Orai1 (B), and SERCA (C) in EDL muscle homogenates from WT and CASQ1-null mice. For Orai1 expression, EDL muscles from constitutive, muscle-specific Orai1 KO (cOrai1 KO) mice were used as a control for validation of the Orai1 antibody employed. (D–G) Relative band intensities (normalized to glyceraldehyde 3-phosphate dehydrogenase antibody [GAPDH]) for each of the proteins shown in A. Data are shown as mean ± SEM; *, P < 0.05. Numbers in bars (n) indicate the number of mice analyzed.