Figure S7.
Rapamycin-inducible PJ-Sac1 recruitment to the Golgi.(A) Schematic of a cell expressing soluble PJ-Sac1 and Golgi-anchored giantin-FRB. Rapamycin, by cross-linking FKBP and FRB, recruits PJ-Sac1 to the Golgi, where it dephosphorylates PtdIns4P to PtdIns. (B) Confocal images confirming PJ-Sac1 translocation from the cytoplasm to the giantin-FRB–enriched Golgi after 10 min with 1 µM rapamycin. Right: GFP-tagged OSH1, a PtdIns4P indicator, reports a reduction of Golgi PtdIns4P. Scale bar, 10 µm.

Rapamycin-inducible PJ-Sac1 recruitment to the Golgi. (A) Schematic of a cell expressing soluble PJ-Sac1 and Golgi-anchored giantin-FRB. Rapamycin, by cross-linking FKBP and FRB, recruits PJ-Sac1 to the Golgi, where it dephosphorylates PtdIns4P to PtdIns. (B) Confocal images confirming PJ-Sac1 translocation from the cytoplasm to the giantin-FRB–enriched Golgi after 10 min with 1 µM rapamycin. Right: GFP-tagged OSH1, a PtdIns4P indicator, reports a reduction of Golgi PtdIns4P. Scale bar, 10 µm.

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