Figure S3. Comparison of PtdIns(4,5) P 2... | Rockefeller University Press

Figure S3.

Comparison of PtdIns(4,5)P2 regeneration during stimulation with different muscarinic agonists and receptors.(A) Time course of homologous nFRETeff between CFP- and YFP-tagged PHPLCδ1 domains during application of carbachol (light blue bar, CCh) for 10 min. Cells were transfected with the probes with or without (−) M1R. (B) Mean of ΔnFRETeff, nFRETeff after 10-min CCh stimulation, and the time constant of nFRETeff regeneration. 10 µM, n = 4 cells; 100 µM, n = 12; and 1,000 µM, n = 5. (C) Time course of nFRETeff during application of 10 µM Oxo-M (gray bar) to cells expressing M5R. (D) Summary of ΔnFRETeff, nFRETeff at 10 min, and the time constant of nFRETeff regeneration for cells expressing M5R. n = 5 cells. *, P < 0.05. n.s. not significant.

Comparison of PtdIns(4,5)P₂ regeneration during stimulation with different muscarinic agonists and receptors. (A) Time course of homologous nFRET_eff between CFP- and YFP-tagged PH_PLCδ1 domains during application of carbachol (light blue bar, CCh) for 10 min. Cells were transfected with the probes with or without (−) M₁R. (B) Mean of ΔnFRET_eff, nFRET_eff after 10-min CCh stimulation, and the time constant of nFRET_eff regeneration. 10 µM, n = 4 cells; 100 µM, n = 12; and 1,000 µM, n = 5. (C) Time course of nFRET_eff during application of 10 µM Oxo-M (gray bar) to cells expressing M₅R. (D) Summary of ΔnFRET_eff, nFRET_eff at 10 min, and the time constant of nFRET_eff regeneration for cells expressing M₅R. n = 5 cells. *, P < 0.05. n.s. not significant.