Figure S2. PtdIns(4,5) P 2 regeneration... | Rockefeller University Press

Figure S2.

PtdIns(4,5)P2 regeneration followed initial depletion by receptor stimulation.(A) Time course of PtdIns(4,5)P2 indicator homologous nFRETeff during treatment (gray bar) with different Oxo-M concentrations in cells overexpressing M1R. The black line is for a control cell without M1R overexpression (M1R (−)). (B) Mean of ΔnFRETeff, final nFRETeff after 10 min in Oxo-M, and the time constant of nFRETeff regeneration during Oxo-M application. 1 µM, n = 7 cells; 10 µM, n = 8; and 100 µM, n = 6. **, P < 0.005. n.s. not significant.

PtdIns(4,5)P₂ regeneration followed initial depletion by receptor stimulation. (A) Time course of PtdIns(4,5)P₂ indicator homologous nFRET_eff during treatment (gray bar) with different Oxo-M concentrations in cells overexpressing M₁R. The black line is for a control cell without M₁R overexpression (M₁R (−)). (B) Mean of ΔnFRET_eff, final nFRET_eff after 10 min in Oxo-M, and the time constant of nFRET_eff regeneration during Oxo-M application. 1 µM, n = 7 cells; 10 µM, n = 8; and 100 µM, n = 6. **, P < 0.005. n.s. not significant.